NASC

NASC ID: N849310

Name: WiscDsLox233237_03B

ABRC stock number: CS849310

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox233237_03B.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G30390.1-P355 and AT4G30400.1-P355 (at chr 4 pos 14865376 (C/14865376-14865455) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 84-100 bps onto the T-DNA. Pools P 0, R 3, C 0 N 0. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453283.

Wiscseq_DsLox233237_03B.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G50430.1 (chr 1 pos 18683017 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG795907.

Wiscseq_DsLox233237_03B.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G50640.1-P355 and AT1G50650.1 (chr 1 pos 18761514 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795908.

Wiscseq_DsLox233237_03B.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G44900.1 (chr 3 pos 16389635 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795909.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox233237_03B

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G50430 AT1G50640 AT1G50650 AT3G44900 AT4G30390 AT4G30400